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. 2001 Mar 5;152(5):971–984. doi: 10.1083/jcb.152.5.971

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of PSGAP at the cell periphery in 10T1/2 fibroblasts. 10T1/2 cells were fixed with 4% paraformaldehyde for 20 min, blocked with 10% BSA, and immunostained with indicated antibodies. (A) Immunostaining using antibodies against PSGAP or antibodies preabsorbed with the GST-PSGAP antigen. (B) Coimmunostaining with anti–PSGAP or anti–PYK2 and antipaxillin antibodies. PSGAP and PYK2 were visualized by FITC-conjugated secondary antibodies, whereas paxillin appeared in red with rhodamine-conjugated secondary antibodies. (C) Immunostaining of PSGAP wild-type (PSGAP-WT), NH₂-terminal–deleted PSGAP (PSGAPΔN), and PH-domain–deleted PSGAP (PSGAPΔPH) in transfected 10T1/2 fibroblasts. Cells were transfected with the indicated constructs and stained with anti–PSGAP antibodies. Bar, 50 μm.