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. 2001 Mar 5;152(5):971–984. doi: 10.1083/jcb.152.5.971

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of PSGAP and Graf mRNAs in various tissues. Northern blot analyses were carried out using a multiple tissue blot (CLONTECH Laboratories, Inc.). Mouse cDNA fragments (796–1,358 bp for PSGAP and 1–717 bp of KIAA0621 for Graf) were labeled with [³²P-α]dCTP by the random prime method and hybridized with the blot. A major transcript at 3.8 kb was detected for PSGAP (top, solid arrow). In addition, two more PSGAP transcripts, at 6 and 8 kb, respectively, were detected (top, open arrows). Graf transcript was detected at ∼9 kb (bottom, arrow). Molecular weight markers are indicated (left).