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. 2001 Mar 5;152(5):985–996. doi: 10.1083/jcb.152.5.985

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The effect of FFA-1 depletion on DNA replication and RPA focus formation. (A) Western blot for FFA-1 in the extracts depleted with either anti–FFA-1 antibody or control antibody. Arrowhead indicates the position of FFA-1. (B) DNA synthesis in nuclei reconstituted around sperm chromatin with the depleted extracts. Samples were taken at the times indicated. (C and D) Immunofluorescence staining for RPA on sperm chromatin incubated in either FFA-1–depleted (C) or control-depleted cytosol (D).

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