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. 2001 Mar 5;152(5):985–996. doi: 10.1083/jcb.152.5.985

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© 2001 The Rockefeller University Press

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Formation of hybrid foci. (A–F) Nuclei were reconstituted around sperm chromatin for 52 min in the presence of GST-Xho and stained with the affinity-purified anti-GST (A and D), RPA (B), or FFA-1 (E). (C) Merge of GST staining and RPA staining. (F) Merge of GST staining and FFA-1 staining. The anti–FFA-1 antibody is against the COOH-terminal end of FFA-1 and does not react with GST-Xho. (G–O) Nuclei reconstituted in the presence of GST-Stu/Xho (G–I), GST-Stu (J–L), and GST (M–O) for 52 min. (G, J, and M) GST staining (green). (H, K, and N) RPA staining (red). (I, L, and O) Merge of GST and RPA staining.

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