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. 2001 Mar 5;152(5):1007–1018. doi: 10.1083/jcb.152.5.1007

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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ILK is required for coupling the actin cytoskeleton to the plasma membrane. Visualization of the plasma membrane (green) and actin filaments (red) of the muscles in embryos that are wild type (a), lack ILK [ilk ¹/Df(3L)Pc-14d] (b), or lack the PS2 integrin (if ^B4/Y) (c). Lateral views are shown of the ventral longitudinal muscles of embryos at stage 17 (a and b) or 16 (c). The detachment of actin from the plasma membrane in the absence of ILK is shown by an arrow in b, and the detachment of the muscle plasma membrane is shown by dashed arrows in b and c. The plasma membrane is labeled with Src-GFP (green) and the filamentous actin with rhodamine-labeled phalloidin (red). Bar, 10 μm.