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. 2001 Mar 5;152(5):1045–1056. doi: 10.1083/jcb.152.5.1045

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Antibody and gold binding to microfibrils extended by surface tension. (A) Fibrillin-rich microfibrils were extended by surface tension, and then labeled with 5-nm colloidal gold and negatively stained. (B) Negatively stained images of microfibrils labeled with antibody12A5.18, either untensioned or extended by surface tension. Only extension up to ∼10 nm is tolerated before the antibody banding pattern is lost. Microfibril direction is indicated by a white arrow, bead position by green arrows, and antibody position is represented by blue arrows.