Figure 2.

CDV rings contain markers for the ER. a–d, BHK cells were transfected with cav^DGV for 24 h, and were then labeled with a polyclonal antibody to PDI (a marker for the ER) and an mAb to the HA tag. PDI (green) was located in the reticular network characteristic of the ER (b) and in some vesicular structures that colocalized to some extent with CDV rings (red) (b and c, arrows). The colocalization between both proteins was corroborated in frozen sections of cav^DGV-transfected cells (d) labeled with the same antibodies, followed by anti-rabbit antibodies conjugated to 15-nm gold (arrows) and anti-mouse antibodies conjugated to 5-nm gold (arrowheads). e–g, BHK cells were double transfected with CFP-tagged ER marker and HA-tagged cav^DGV. The cells were labeled with a polyclonal antibody to GFP (to increase the signal) and the mAb to HA tag (e). Clear colocalization could be observed between some of the CDV rings (red) and the ER marker (green) (f and g, arrows). Bars: (d) 100 nm; (a–e) 5 μm.