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. 2001 Mar 5;152(5):877–894. doi: 10.1083/jcb.152.5.877

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Endogenous FTCD localizes to Golgi elements and to vimentin IFs. (A) COS-7 cells were processed for immunofluorescence using monoclonal anti-FTCD (FTCD) and polyclonal anti-GM130 (GM130) antibodies. FTCD colocalizes with the Golgi marker GM130 but is also present in areas lacking GM130 (arrowheads). (B) NIH3T3 cells stably expressing GFP–vimentin were processed for immunofluorescence using monoclonal anti-FTCD antibodies. FTCD localizes to Golgi elements (arrows) and to vimentin filaments (arrowheads). (C) COS-7 cells were processed for immunofluorescence using polyclonal anti-FTCD and monoclonal anti-vimentin antibodies. FTCD localizes to vimentin filaments in cell periphery (arrowheads).