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. 2001 Mar 5;152(5):877–894. doi: 10.1083/jcb.152.5.877

Figure 4.

Figure 4

FTCD remodels the vimentin IF cytoskeleton independent of the actin and the microtubule cytoskeletons. COS-7 cells (A–D) or NRK cells (E) were transfected with rFTCD–pcDNA, grown for 48 h, and processed for immunofluorescence. (A) Cells were labeled with polyclonal anti-FTCD and monoclonal antivimentin antibodies. The border of a transfected cell is outlined by dashed lines. The vimentin cytoskeleton is perturbed in the transfected cell colocalizes with vimentin to thick fibers originating from the Golgi region. (B) Cells were labeled with polyclonal anti-FTCD antibodies and phalloidin–FITC. Actin distribution in the transfected cell is indistinguishable from that in untransfected cells. (C) Cells were labeled with polyclonal anti-FTCD and monoclonal anti–β-tubulin antibodies. The microtubule pattern in transfected cells is indistinguishable from that in untransfected cells. (D) Cells were treated with 10 μg/ml nocodazole for 3 h at 37°C, then labeled with polyclonal anti-FTCD and monoclonal anti–β-tubulin antibodies. FTCD fibers persist despite the disruption of the microtubular network. (E) Cells were treated with 10 μg/ml nocodazole for 3 h at 37°C, and then labeled with polyclonal anti-FTCD and monoclonal antivimentin antibodies. FTCD/vimentin fibers persist despite the disruption of the microtubular network.