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. 2001 Mar 5;152(5):877–894. doi: 10.1083/jcb.152.5.877

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Golgi elements interact with vimentin filaments in vivo. (A) MFT-6 cells were labeled with polyclonal anti-GM130 and monoclonal antivimentin antibodies. Extended Golgi elements align with vimentin filaments (arrowheads). (B) MFT-6 cells were treated with 10 μg/ml nocodazole for 5 h at 37°C before labeling as in A. The Golgi complex was disrupted into Golgi ministacks, but the Golgi ministacks were associated with vimentin filaments (arrowheads). Arrow points to a mass of collapsed vimentin. (C) NRK cells were treated with 10 μg/ml nocodazole for 5 h at 37°C and then labeled with polyclonal anti-GM130 and monoclonal anti-FTCD antibodies. FTCD colocalizes with GM130 in Golgi ministacks (arrowheads).