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. 2001 Oct 15;155(2):261–270. doi: 10.1083/jcb.200104094

Figure 2.

Figure 2.

Polarized recruitment of Bee1p downstream of Cdc42p activation. (A) Polarization of Bee1-GFP in cells released from a cdc28ts-induced G1 arrest in the presence of Lat-A. (B) Quantitation of Bee1-GFP polarization and its temporal relationship to bud emergence. □ show percentage of cells with a new bud in the presence of DMSO, and ○ show percentage of cells with polarized Bee1-GFP in the presence of Lat-A. (C) Bee1-GFP polarization was examined in G1-arrested cdc28-13 cells expressing the CDC42L61-HA allele under the control of the GAL1 promoter in the presence of Lat-A. Immunofluorescence analysis shows colocalization of Bee1-GFP and Cdc42L61-HA.