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. 2001 Oct 15;155(2):261–270. doi: 10.1083/jcb.200104094

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Copyright © 2001, The Rockefeller University Press

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Figure 6. — Vrp1p is required for recruitment of Bee1p to the presumptive bud site. (A) The vrp1ΔC and bee1ΔWH1 mutations abrogate the Vrp1p–Bee1p interaction. Vrp1-myc was immunoprecipitated from extracts of wild-type cells and cells in which either the WH1 domain of Bee1p (bee1ΔWH1) or the COOH terminal 56 amino acid of Vrp1p (vrp1ΔC) was deleted. The first two lanes in the left panel and both lanes in the right panel show that a similar level of the wild-type and mutant proteins were present in the extracts. (B, a) Polarization of Bee1-GFP in cells released from G1 in the presence of Lat-A was examined in wild-type and vrp1ΔC cells. Bee1-GFP was visualized by staining with an anti-GFP antibody. (b) Polarization of Vrp1-GFP in cells released from G1 in the presence of Lat-A was examined in wild-type and bee1ΔWH1 cells. Vrp1-GFP was visualized by staining with an anti-GFP antibody. Bar, 5 μm.