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. 2001 Oct 15;155(2):279–290. doi: 10.1083/jcb.200102106

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Copyright © 2001, The Rockefeller University Press

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Figure 4. — Late states of fusion pores. (A) LSM images of fused LBs at the cell periphery before (a) and at different times (b–d, corresponding to the asterisks in B; bleaching was at time 0) after a FRAP measurement. (B) Minor changes in I_{FM 1–43} recovery rates of LBs 1, 2, and 4, measured at ∼10, 20, and 30 min after initial fusion. (C) Mean time constants of individual LBs (n = 8 ± SD) after repeated FRAP measurements. (D) Progressive increase in I_{FM 1-43} recovery of LB no. 3 in A within three subsequent measurements. Data were fit by Eq. A4 (solid lines). Arrow denotes transition of the fusion pore diameter (see text). Bar, 10 μm.