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. 2001 Oct 15;155(2):279–290. doi: 10.1083/jcb.200102106

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Copyright © 2001, The Rockefeller University Press

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Figure 8. — SEM and TEM of stimulated cells. (A) Surface structures of two adjacent cells (*, cell border), fixed 10 min after stimulation by ATP. Manifold LB protrusions are visible out of a cluster of differently sized pores. Even small structures could be interpreted as fusion pores (arrow) considering their distinct appearance in comparison to specimen preparation artifacts (arrowheads). (B) Prominent LB release out of a fused vesicle. (C) Compound exocytosis and LB release visualized by rapid freezing/freeze substitution/TEM. Arrow indicates membrane transition of two fused vesicles (*, smaller one). In contrast to intracellular LBs (inset), LB appear loosely packed during release. (D) Change in the number of pores per cell exceeding 0.75 μm ∅, 10 min after stimulation by ATP. Bar (inset), 0.5 μm.