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. 2001 Nov 12;155(4):581–592. doi: 10.1083/jcb.200106065

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Copyright © 2001, The Rockefeller University Press

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Figure 1. — Phenotype of cdc42-6 mutants. (A and B) CDC42 (open symbols) and cdc42-6 (closed symbols) cells were grown to a density of about 10⁶ cells/ml in YPD at 25°C and split into flasks that were then incubated either at 25°C (circles) or 33°C (squares). Aliquots were fixed with 3.7% formaldehyde at hourly intervals and cell number (A) and the percentage of cells budded (B) were scored. (C) cdc42-6 cells were sonicated briefly and spread onto YPD plates. The number of cell bodies per microcolony was scored immediately after plating (T = 0) or after 12 h at 23 or 33°C, as indicated. An unbudded cell was counted as one cell body, and a budded cell was counted as two cell bodies.