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. 2001 Nov 12;155(4):581–592. doi: 10.1083/jcb.200106065

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Copyright © 2001, The Rockefeller University Press

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Figure 3. — Trafficking of Bgl2, invertase, and CPY in the cdc42-6 mutant. (A) The graph depicts the percentage of Bgl2 protein that remains internal in CDC42, cdc42-6, and sec6-4 strains. The percent distribution of Bgl2 was determined by immunoblot analysis using affinity-purified antibodies raised against the COOH terminus of Bgl2. Quantitation of the bands was done using ImageQuant software and the results are presented as a percentage of total Bgl2 that is found internally. (B) The graph depicts the percentage of invertase protein that remains internal in CDC42, cdc42-6, and sec6-4 strains. The percent of internal invertase was determined by enzyme assays and then shown in bar graph form. (C) Transport of the vacuolar protein CPY is not affected in cdc42-6, as it is found exclusively in the mature form, (mCPY, 61 kD). Wild-type CDC42 and the late secretory mutant, sec6-4, are also shown to process CPY fully to the mature form while sec18-1, an ER-to-Golgi mutant, accumulates the earlier forms. (D) Vesicle accumulation was quantitated for wild-type and cdc42-6 cells at both permissive and restrictive (33°C for 1 h) temperatures from randomly chosen fields of cells. Numbers shown were determined by dividing the total number of 80–100-nm vesicles observed per field of cells by the total number of cells present per field.