Figure 4.

Snc1, Bgl2, and Gas1 are all associated with vesicles that accumulate in cdc42-6 cells. Vesicle gradients of 20 to 40% sorbitol were prepared for each of CDC42, cdc42-6, and sec6-4. Normalized volumes of lysed cells (shifted for 1 h to 37°C for sec6-4 and 33°C for CDC42 and cdc42-6) for each strain were layered onto a gradient before a 1.5 h, 71,000 g spin. The gradients were then collected in 16 fractions and these fractions were subjected to SDS-PAGE analysis and blotted with the indicated antibodies.