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. 2001 Nov 12;155(4):661–674. doi: 10.1083/jcb.200101099

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Copyright © 2001, The Rockefeller University Press

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Figure 1. — L1 is shed without cytoplasmic tail. (A) CHO or L1-transfected CHO cells were incubated at 37°C in suspension in medium without FCS for 1 h. The cell pellet was lysed and analyzed by Western blot with mAb UJ127.11 to the ectodomain or pcytL1 to the cytoplasmic domain of L1. (B) Schematic representation of L1 cleavage fragments and their relative localization. The location of antibody recognition sites are indicated. (1), mAb 5G3; (2), mAb UJ 127.11; (3) pcytL1.