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Regulated cleavage of L1 is blocked by metalloproteinase inhibitor. Attached AR cells were treated with PMA (100 ng/ml), pervanadate (200 mmolar), or MCD for 1 h at 37°C in the absence or presence of BB-3103 inhibitor. (A) Supernatants were immunoprecipitated with mAb to L1 coupled to Sepharose and analyzed for shed L1 using blotting with mAb UJ127.11 followed by ECL detection. (B) Cell pellets were lysed and blotted with pcytL1 followed by ECL detection.
