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. 2001 Nov 12;155(4):625–636. doi: 10.1083/jcb.200103128

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Copyright © 2001, The Rockefeller University Press

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Figure 3. — The ionic strength dependence of M5 _HMM in vitro motility. Normalized filament velocity (mean ±SEM, n > 10 filaments) as a function of added potassium chloride for M5_HMM expressed with LC1sa (•) and calmodulin (▴) (M5_HMM loading concentration of 10–25 μg/ml). Normalized filament velocity as a function of added potassium chloride for smooth muscle myosin II (♦) from Harris and Warshaw (1992) for comparison (loading concentration of 100 μg/ml). Note the reduction in actin filament velocity at potassium chloride concentrations <125 mM for both M5_HMM and myosin II. Also note the plateau at physiological ionic strengths and greater (between 125 mM and 325 mM potassium chloride) for M5_HMM. Velocities were normalized to the velocity at 25 mM potassium chloride. A similar dependence of actin filament velocity was observed regardless of light chain content.