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Immunofluorescent staining of Sec6 in cells following transient expression of PKD-K618N. NRK-52E and MDCK cells were transiently transfected with plasmid encoding GFP-PKD-K618N according to Materials and methods. MDCK cells were subsequently incubated at 19°C for 2 h. Cells were fixed, permeabilized, and stained for Sec6 (mAb 9H5). Bar, 5 μm.
