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. 2001 Nov 12;155(4):637–648. doi: 10.1083/jcb.200105081

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Copyright © 2001, The Rockefeller University Press

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Figure 6. — S. aureus induces activation of the initiator caspases 8 and 9. (A) Jurkat cells were incubated with the indicated dilutions of Wood 46 supernatant (SN). After 4 h, cell extracts were prepared and analyzed for caspase activity using the fluorogenic substrates IETD AMC for caspase 8 (▪) and LEHD AMC for caspase 9 (•). The two dashed lines represent IETDase and LEHDase activity in untreated cells. (B) Western blot analysis of the status of caspase-8 (top) and caspase-9 (bottom) in Jurkat cells that were either left untreated (control) or incubated for 4 h with the indicated dilutions of Wood 46 supernatant (SN). The uncleaved proforms and the p43/p41 fragments of caspase-8 and the p37 fragment of caspase-9 are indicated.