Fig. 4.
Representative Western blots from three experiments showing (A) the bands of p-JNK-1/p-JNK-2, total JNK-1/JNK-2, and quantified ratios (mean±S.D.) of the band densities of p-JNK1/JNK1 and p-JNK2/JNK2; and (B) the bands of p-ERK-1/p-ERK-2, total ERK-1/ERK-2, and quantified ratios (mean±S.D.) of p-ERK1/ERK1 and p-ERK2/ERK2 in primary human limbal epithelial cultures exposed to normal (312 mOsM) and hyperosmolar media (500 mOsM) created by adding 90 mM NaCl with or without 20 μM SB202190 (SB), 40 μM PD98059 (PD), 1 μM dexamethasone (Dex) or 10 μg mL−1 doxycycline (Doxy). *P < 0.05, **P < 0.01, n = 3, compared with the levels stimulated by the hyperosmolar media.