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. 2008 Jan 23;3(1):e1487. doi: 10.1371/journal.pone.0001487

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Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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NIH3T3 cells were transfected with wild type or mitochondrial localization-defective MULAN mutants, together with MT-RFP. C339A is the RING finger-mutant control. Cells were fixed 24 h post-transfection and the percentage of RFP-positive cells with perinuclear-clustered mitochondria was determined.