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. 1997 Jul 7;186(1):121–129. doi: 10.1084/jem.186.1.121

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Chemical cross-linking of wild-type IL-5 and IL-5 (E12K) to IL-5 receptors. COS cells transfected with human IL-5 receptor α and β_c chain cDNAs were incubated with ∼70 nM ¹²⁵I-IL-5 or ¹²⁵I-IL-5 (E12K) for 2 h at 4°C in the presence or absence of a 100-fold molar excess of unlabeled IL-5, then cross-linked with 1 mM BS³. After detergent lysis of cells, soluble extracts were analyzed by SDS-PAGE and radiolabeled bands visualized by a PhosphoImager.