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Wild-type IL-5 but not IL-5 (E12K) stimulates tyrosine phosphorylation of a 150-kD protein in human eosinophils. Human eosinophils were stimulated with the indicated concentrations of cytokines for 5 min at 37°C. Cells were pelleted, resuspended in SDS sample buffer and lysates were electrophoresed and immunoblotted with anti-phosphotyrosine antibody.
