Figure 1.

Anergy of enriched CD4⁺ T cells from SEA-treated transgenic mice. Tg CD4⁺ cells were broadly unresponsive to antigenic restimulation in vitro after in vivo exposure to SEA. [³H]thymidine incorporation by CD4⁺ T cells stimulated with (a) 5 μM PCCF and APCs, (b) increasing concentrations of PCCF and APCs, and (c) other T cell mitogens. (d) Cytokine production by CD4⁺ T cells was also decreased by the in vivo SEA treatment. Similar results were seen in at least three repeated experiments.