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. 1997 Jul 7;186(1):71–81. doi: 10.1084/jem.186.1.71

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Anti–IFN-γ reverses SEA-induced anergy. Treatment with anti–IFN-γ Ab increased CD4⁺ T cell recovery and viability after SEA treatment. (a) CD4⁺ T cells from SEA-treated and control AND mice were cultured with neutralizing concentration of anti–IFN-γ Ab (XMG1.2). Live cell recovery on day 4 is shown. Cells recovered from above cultures were analyzed for live cell content. (b) Percentage of live cells as determined by F/S. (c) Percentage of CD4⁺ cells that stained positively with PI. Gated populations of CD4⁺ cells are shown.

Anti–IFN-γ reverses SEA-induced anergy. Treatment with anti–IFN-γ Ab increased CD4⁺ T cell recovery and viability after SEA treatment. (a) CD4⁺ T cells from SEA-treated and control AND mice were cultured with neutralizing concentration of anti–IFN-γ Ab (XMG1.2). Live cell recovery on day 4 is shown. Cells recovered from above cultures were analyzed for live cell content. (b) Percentage of live cells as determined by F/S. (c) Percentage of CD4⁺ cells that stained positively with PI. Gated populations of CD4⁺ cells are shown.