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. 1997 Jul 7;186(1):159–164. doi: 10.1084/jem.186.1.159

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Detection of transgene IL-10 mRNA in T10.11 clone cells. An RNase protection assay (RPA) was used for distinguishing endogenous and transgene mRNA. (a) The DNA construct used to generate the RNA probe for measuring IL-10 expression. The RNA probe is represented by an arrow and protected RNase digestion products are shown. (b) RNase-digestion products after hybridization to ³²P-labeled RNA probe. Lane 1, yeast RNA; lane 2, RNA from rested T10.11 clone cell 11 d after activation with PLP 139–151; lane 3, RNA from activated T10.11 clone cells 48 h after activation with PLP 139–151; lane 4, spleen RNA.