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. 1997 Jul 21;186(2):279–288. doi: 10.1084/jem.186.2.279

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Flow cytometric analysis in v-rel/ikba transgenic mice. (A) Flow cytometric analysis of CD4 and CD8 surface markers in thymocytes from 6-wk-old ikba (a and c) and v-rel/ikba (b and d) transgenic mice. (B) Flow cytometric analysis of surface markers in splenocytes from 6-mo-old ikba (a, c, e, and g) and v-rel/ikba (b, d, f, and h) transgenic mice. Flow cytometry was performed using a flow cytometer (Epics Profile II) and cell sorter (Coulter Corp.). Single cell suspensions from thymocytes and splenocytes were prepared and analyzed for surface markers expression of CD4, CD8, HSA, TCR-α/β, B220, Mac-1, and Gr-1 as previously described (8).