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. 1997 Jul 21;186(2):269–278. doi: 10.1084/jem.186.2.269

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Specificity of NP fixation on Cl.13 cell line. (A) Dose-dependent binding of biotinylated NP. The median intensity value of the fluorescence histograms are indicated. FITC-conjugated streptavidin was used for the detection of NP binding. (B) Competition between unlabeled NP and biotinylated NP. The Cl.13 cell line was incubated with 2 μg of biotinylated NP and various amounts of unlabeled NP. Percentages of biotinylated NP binding detected as above was calculated as indicated in Materials and Methods.