Table 2.
Stimulation | NP | Ig synthesis | ||||||
---|---|---|---|---|---|---|---|---|
IgG | IgM | IgA | ||||||
μg/ml | ||||||||
− | − | 1.0 | 1.4 | <0.2 | ||||
αIg | − | 2.9 | 0.2 | 0.3 | ||||
αIg + IL-2 + IL-10 | − | 7.7 | 18.7 | 0.4 | ||||
αIg + IL-2 + IL-10 | + | 2.9 (62) | 7.1 (62) | <0.2 (>50) | ||||
CD40L | − | 2.9 | 7.3 | 0.3 | ||||
CD40L + IL-2 + IL-10 | − | 89.5 | 73.7 | 12.7 | ||||
CD40L + IL-2 + IL-10 | + | 43.4 (52) | 36.6 (50) | 6.5 (49) |
B cells were stimulated with either an anti-Ig antibody or a CD40L transfectant in combination with IL-2 and IL-10, with or without an optimal dose (30 ng/ml) of NP. Ig synthesis was evaluated in 12-d culture supernatants. Values in brackets indicate the percentage of inhibition calculated as indicated in Materials and Methods. Standard deviations never exceeded 10% of the mean value.