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. 1997 Jul 21;186(2):307–312. doi: 10.1084/jem.186.2.307

Figure 1.

Figure 1

(A) Interleukins in the supernatants of MBP-specific Th1 and Th2 cultures at the day of injection. (Gray bars) Th1 cultures; (black bars) Th2 cultures. (B) Both Th1 and Th2 anti-MBP T cells induce EAE in RAG-1 KO recipients. Mice were injected intravenously with 5 × 106 Th1 cells (open squares, n = 9), 5 × 106 Th2 cells (filled squares, n = 6), 0.2 × 106 Th1 cells (open circles, n = 5), or 0.2 × 106 Th2 cells (filled circles, n = 3). Data is presented as a percentage of maximum possible EAE that would be reached if all the mice were at level 5. Animals reaching level 5 were sacrificed and their score was kept at level 5 for the remaining of the experiment. Data from one representative experiment (out of five) is shown. (C) Coinjection of Th2 does not alter the kinetics of EAE caused by Th1 cells. RAG-1 KO recipient mice were injected intravenously with 10 × 106 Th1 cells (open squares, n = 12), 10 × 106 Th2 cells (filled squares, n = 12), or a mixture of 5 × 106 Th1 cells and 5 × 106 Th2 cells (cross, n = 4). (D) MBP-specific Th2 cells do not induce EAE in syngeneic immunocompetent recipients. Ten million Th1 cells (open symbols) or Th2 cells (filled symbols) were injected intravenously into RAG-1 recipients (squares, n = 12 for both Th1 or Th2 recipients) or B10.PL recipients (triangles, n = 6 for both Th1 and Th2 recipients).