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. 1997 Jul 21;186(2):307–312. doi: 10.1084/jem.186.2.307

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Th2 cells but not Th1 cells are recovered from animals to which cells from Th2 cell cultures had been injected. Spleen (top two panels) and CNS cells from mice injected with Th2 cells were prepared 40 d after injection, when mice were at level 2 and 3 of EAE. The lower two panels show purified CD4-positive cells. RT-PCR was performed to determine mRNA levels of IL-4, IFN-γ, and IL-10. The IFN-γ mRNA present in total splenocytes and absent in CD4 purified splenocytes and lymphocytes from CNS is likely coming from NK cells.