Figure 3.

HSF1 granules are transient and reversible structures. Time-lapse microscopy during heat shock and recovery on living HeLa cells transiently transfected with a HSF1–GFP construct. Images were acquired before heat shock (a), after 30 seconds (b), 1 minute (c), and 3 minutes (d) of heat shock at 42°C, 2 minutes of recovery (e), 8 minutes of recovery (f), 40 minutes of recovery (g) and after 30 seconds (h), 1 minute (i), and 3 minutes (j) of a second heat shock at 42°C. (Bar = 5 μm.)