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Kinetics of HSF1 granule formation during azetidine and cadmium treatments. Living HeLa cells transiently expressing a HSF1–GFP construct were exposed to 30 μM cadmium or to 5 mM azetidine, and images were acquired after 5 minutes (a), 10 minutes (b), 16 minutes (c), and 24 minutes (d) of cadmium treatment and after 20 minutes (e), 25 minutes (f), 37 minutes (g) and 45 minutes (h) of azetidine treatment. (Bars = 5 μm.)
