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. 1997 Aug 4;186(3):365–373. doi: 10.1084/jem.186.3.365

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(A and B) Anti-NOS2 immunoblot of tissue and cell extracts from NOS2-modified MRL–lpr/lpr mice. 20-wk-old mice were examined. Extracts from kidney, spleen, and liver of (+/+), (+/−) , (−/−) and BALB/c (B) mice are displayed in A, and that for peritoneal macrophages in B. For negative and positive controls, we used extracts from the mouse macrophage cell line J774 without (0) or with LPS and IFN-γ (LI). The predominant band appeared at a region corresponding to about 130 kD.

(A and B) Anti-NOS2 immunoblot of tissue and cell extracts from NOS2-modified MRL–lpr/lpr mice. 20-wk-old mice were examined. Extracts from kidney, spleen, and liver of (+/+), (+/−) , (−/−) and BALB/c (B) mice are displayed in A, and that for peritoneal macrophages in B. For negative and positive controls, we used extracts from the mouse macrophage cell line J774 without (0) or with LPS and IFN-γ (LI). The predominant band appeared at a region corresponding to about 130 kD.