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. 1997 Aug 4;186(3):375–383. doi: 10.1084/jem.186.3.375

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SPE-C and SEA induce homotypic aggregation in LG-2 cells. Freshly split LG-2 cells were incubated with 1 μg/ml of recombinant toxin in 24-well culture plates and monitored for 24 h. Percent aggregation was determined by counting the number of cells not bound to an aggregated clump.