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. 1997 Aug 4;186(3):375–383. doi: 10.1084/jem.186.3.375

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Reversibility of SPE-C dimer formation. Recombinant SPE-C (2 mg/ml) was dialyzed overnight in (A) 10 mM BisTris-Tris, pH 6.0, or (B) 10 mM BisTris-Tris, pH 9.0. Samples (20 μl) from each dialysis were incubated for 1 h at room temperature in 100 μl 50 mM BisTris-Tris–0.1 M NaCl at either pH 6.0, 7.0, 8.0, or 9.0, and then analyzed by size exclusion chromatography. The exact retention time of each peak was determined with the Perceptive Biocad analysis software and is shown beside each peak. Each trace represents absorbance at 280 nm. The resulting figure was generated from the Perceptive Biocad program using the stacked trace mode. The small peak to the right of the pH 6.0 generated dimer is not monomer, but an SPE-C cleavage product also seen to the right of the pH 9.0 monomer peak.