Table 2.
Upper compartment | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Lower compartment | 0 | 0.004 | 0.02 | 0.1 | 0.05 | |||||
μg/ml | μg/ml | μg/ml | μg/ml | μg/ml | μg/ml | |||||
0 | 1.0 ± 0.09 | 1.44 ± 0.38 | 10.68 ± 1.44 | 18.1 ± 2.21 | 11.3 ± 1.17 | |||||
0.004 | 2.04 ± 0.16 | 1.93 ± 0.15 | 5.68 ± 0.62 | 12.92 ± 1.38 | 11.22 ± 0.61 | |||||
0.02 | 5.14 ± 0.27 | 4.07 ± 0.42 | 15.39 ± 1.27 | 14.36 ± 1.15 | 10.62 ± 0.14 | |||||
0.1 | 6.25 ± 0.11 | 5.17 ± 0.43 | 16.09 ± 0.73 | 15.12 ± 1.88 | 12.78 ± 1.75 | |||||
0.5 | 8.64 ± 0.65 | 6.87 ± 0.80 | 5.97 ± 1.02 | 8.83 ± 0.34 | 6.85 ± 0.71 |
Cathepsin G was added into upper and lower wells of chemotaxis chamber together with monocytes. Results are expressed as chemotaxis index ± standard error of migration. Migration in control buffer was 16.2 ± 1.5 cells HPF. Chemotaxis indexes equal or higher than two are statistically significant in comparison to migration of T cells to media alone.