Table 3.

Histological Evaluation of Cellular Infiltration after Subcutaneous Injection of Cathepsin G in BALB/c Mice

						Dermis				Subcutaneous
Animal No.		Injection		μg		PMN		MNC		PMN		MNC
1		PBS				–		–		–		–
2		PBS				–		–		–		–
3		PBS				–		–		–		–
4		CathG		0.005		–		–		1F		1F
5		CathG		0.005		1F		–		1F		–
6		CathG		0.005		–		–		–		1F
7		CathG		0.05		1F		–		2F		1F
8		CathG		0.05		1F		1F		1F		1F
9		CathG		0.05		–		–		–		–
10		CathG		0.5		1F		2MF		2F		2MF
11		CathG		0.5		–		1F		–		1MF
12		CathG		0.5		2F		2MF		3MF		2F
13		CathG		5.0		–		–		–		1F
14		CathG		5.0		–		1MF		1MF		1F
15		CathG		5.0		1F		1F		2F		2F
16		Boiled CathG		0.5		–		–		–		–
17		Boiled CathG		0.5		–		–		–		–
18		Boiled CathG		0.5		–		1F		–		1F

Mice received a single injection of various doses of either cathepsin G (CathG; Calbiochem Corp.) boiled cathepsin G in 0.2 ml of PBS, or PBS alone subcutaneously. At 24 h after the injection, the injection site of skin was excised and fixed in 10 % phosphate-buffered formalin and stained with hematoxylin–eosin stain for histological examination. Grading as follows: 1, minimal; 2, mild; 3, moderate. –, No significant lesion; F, focal; MF, multifocal.