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. 1997 Aug 29;186(5):767–775. doi: 10.1084/jem.186.5.767

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Vβ8.1/8.2 versus Vβ-pool staining of thymocytes and lymph node cells from a wild-type C57BL6 (WT [B6]) and a pTα-deficient (pTα^−/−) mouse (four-color analysis). Thymocytes or lymph node cells were stained with an antibody mix containing CD3–biotin, Vβ8.1/8.2– FITC, and nine distinct PE-conjugated anti-Vβ antibodies (anti-Vβ2, 3, 4, 5.1/5.2, 6, 7, 9, 10b, 13). CD3⁺ cells were detected with a streptavidin–Tricolor reagent. Before acquisition, PI was added. (Top) Dot blots of cells that have passed three gates: the FSC/SSC gate for live cells, the gate for PI⁻ cells, and the pulse-width gate that eliminates cell doublets (data not shown). (Bottom) The cells depicted have passed in addition the gate for CD3^high cells shown in the top.