Table 3.
Lymphocyte Populations in Irradiated wt Mice Reconstituted with TC-PTP +/+ or −/− BM
| Donor BM | Organ | Cellularity (×106 ± SE) | Absolute No. of different cell populations (×106 ± SE) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD4+8+ | CD4+ | CD8+ | B220+sIgM− | B220+sIgM+ | ||||||||||
| +/+ | Thymus | 40.5 ± 11.3 | 29.6 ± 8.2 | 6.7 ± 2.2 | 1.8 ± 0.6 | NA | NA | |||||||
| −/− | Thymus | 60.3 ± 14.8 | 31.9 ± 4.2 | 11.7 ± 3.2 | 4.1 ± 1.1 | NA | NA | |||||||
| +/+ | BM | 9.6 ± 1.6 | NA | NA | NA | 0.44 ± 0.07 | 0.27 ± 0.05 | |||||||
| −/− | BM | 5.7 ± 0.7 | NA | NA | NA | 0.37 ± 0.04 | 0.14 ± 0.02 | |||||||
| +/+ | Spleen | 83.8 ± 9.9 | NA | 24.2 ± 4.3 | 6.0 ± 1.5 | 21.9 ± 2.4 | 13.4 ± 1.3 | |||||||
| −/− | Spleen | 83.3 ± 12.0 | NA | 14.7 ± 1.7 | 3.4 ± 0.9 | 27.4 ± 5.4 | 12.6 ± 2.4 | |||||||
| +/+ | LN | 8.4 ± 1.8 | NA | 2.7 ± 0.6 | 0.59 ± 0.1 | 0.58 ± 0.18 | 0.39 ± 0.12 | |||||||
| −/− | LN | 11.9 ± 3.1 | NA | 2.3 ± 0.1 | 0.6 ± 0.1 | 1.5 ± 0.1 | 0.5 ± 0 | |||||||
All wt recipients were irradiated with 900 rads of γ irradiation 18 h before an intravenous injection of 1.5 × 106 BM cells. Recipients were killed 2 mo after the BM transplantation. Data are presented as the mean of 3–4 mice/group. NA, not available.