Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Aug 29;186(5):749–756. doi: 10.1084/jem.186.5.749

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Edema formation in the reverse Arthus reaction in the skin. 25 μg of rabbit anti–chicken egg albumin IgG was injected i.d. followed by 20 mg/kg body weight i.v. chicken egg albumin together with 100 μl Evans blue dye (6.25 mg/ml). Dorsal skins were harvested from C5aR (−/−) mice (striped bars) and their wild-type littermates (black bars) after 3 h and edema was evaluated as weight of 1 cm² skin sections (A). In addition, edema was quantitated as enhanced microvascular permeability by extraction of extravasated Evan's blue dye with formamide from injected skin sections (B). Ab controls (Ab control) received Ab to chicken egg albumin intratracheally without i.v. injection of chicken egg albumin. Mice treated with PBS intratracheally followed by i.v. chicken egg albumin served as Ag controls (Ag control). Data are represented as mean ± SEM, n = 8–12 animals in each group. *P <0.001 (A), *P <0.0001 (B).

Edema formation in the reverse Arthus reaction in the skin. 25 μg of rabbit anti–chicken egg albumin IgG was injected i.d. followed by 20 mg/kg body weight i.v. chicken egg albumin together with 100 μl Evans blue dye (6.25 mg/ml). Dorsal skins were harvested from C5aR (−/−) mice (striped bars) and their wild-type littermates (black bars) after 3 h and edema was evaluated as weight of 1 cm² skin sections (A). In addition, edema was quantitated as enhanced microvascular permeability by extraction of extravasated Evan's blue dye with formamide from injected skin sections (B). Ab controls (Ab control) received Ab to chicken egg albumin intratracheally without i.v. injection of chicken egg albumin. Mice treated with PBS intratracheally followed by i.v. chicken egg albumin served as Ag controls (Ag control). Data are represented as mean ± SEM, n = 8–12 animals in each group. *P <0.001 (A), *P <0.0001 (B).