Table 1.
Effect of Virus Infection on Infiltration of CD8+ Cells, Tumor Cell Density, and Tumor Size
| RIP (GP × Tag2) | P | RIP-Tag2 | ||||
|---|---|---|---|---|---|---|
| 8 d after LCMV infection | ||||||
| CD8+ cell infiltration (%) | 39.13 ± 8.5 | <0.0001 | 2.59 ± 2.40 | |||
| Tumor cell density (%) | 71.25 ± 10.7 | <0.001 | 92.65 ± 4.39 | |||
| Tumor size (mm2) | 0.25 ± 0.07 | 0.002 | 0.46 ± 0.46 | |||
| Uninfected | ||||||
| CD8+ cell infiltration (%) | 3.88 ± 1.90 | NS | 2.11 ± 1.66 | |||
| Tumor cell density (%) | 94.13 ± 4.01 | NS | 95.11 ± 2.42 | |||
| Tumor size (mm2) | 0.51 ± 0.29 | NS | 0.64 ± 0.20 |
Morphometric histological analysis: CD8+ cell infiltration indicates the percentage of insulinoma square zones (100 × 100 μm each) containing CD8+ cells in relation to the total of square zones (20–30) analyzed per mouse pancreas. Tumor cell density indicates the percentage of areas consisting of viable tumor cells in relation to the total area per individual sample square zone. Tumor size is estimated from measuring the size of the area covered by each insulinoma. Data are mean ± SD from four mice per group, analyzed using one to three nonadjacent pancreas sections revealing four to six insulinomas per mouse. Each insulinoma was analyzed by arbitrarily placing five sample square zones of 100 × 100 μm each. At the time of necropsy, all mice were 79–90 d old. The upper part of the table shows data from mice that had been infected with 2,000 PFU LCMV i.v. 8 d earlier, and the lower part shows data from untreated mice. NS, i.e., P >0.05.