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. 1997 Aug 18;186(4):507–515. doi: 10.1084/jem.186.4.507

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Transgenic TCR-expressing cells are not deleted in p17/ IFA-treated mice. Mice were injected intraperitoneally with 500 μg p17/ IFA (A, C, E, G, I) or PBS/IFA (B, D, F, H, J) on day −14, and with 200 μg p17/CFA subcutaneously and 400 ng pertussis toxin intraperitoneally on day 0. Splenic cells were stained with anti-CD4–PE and anti-Vβ8–FITC antibodies, and analyzed by FACS^®. Cells were analyzed on days −9 (A and B), day −4 (C and D), and day 0, before EAE induction (E and F), as well as day 1 (G and H) and day 5 (I and J) after EAE induction.