Figure 6.
Tolerized cells regain encephalitogenicity upon transfer into tolerogen-free host. TR− mice were injected intraperitoneally with either PBS/IFA or 500 μg p17/IFA. 3 wk later, spleen cells from either p17- or PBS-pretreated mice, containing 3 × 106 Vβ8+CD4+ T cells, were injected intravenously into lethally irradiated (900 rads), RAG-1–deficient bone marrow–reconstituted PL/J mice. 1 d after the spleen cell transfer, EAE was induced as described in Fig. 1. Half of the mice that received the cells from p17/IFA-pretreated donors also received 500 μg p17/IFA, intraperitoneally, 5 d after EAE induction. Similar results were obtained in two independent experiments with five to eight mice per group.