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. 1997 Aug 18;186(4):489–495. doi: 10.1084/jem.186.4.489

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Estrogen inhibition of bone resorption by purified rabbit osteoclasts. Purified osteoclasts were incubated with medium (A) lacking or (B) containing 0.1 nM E₂ for 24 h on dentine slices (×40). After removal of osteoclasts, resorption pits formed by osteoclasts were stained with acid hematoxylin and observed under a light microscope. (C) Inhibitory effects of E₂ on osteoclast-mediated bone resorption. Purified osteoclasts were cultured on dentine slices (150 cells/slice) in medium (Con) or in medium containing 0.001–1 nM E₂ (E₂) or 1 nM salmon calcitonin (CT). Osteoclastic bone resorption activity was measured in terms of pit area formed by purified osteoclasts after 24 h of incubation. Pit area per pit number is also indicated (inset). Values are means ± SD, n = 4. *P <0.05, **P <0.01, ***P <0.005 compared with control groups. Data are representatives of those obtained in three additional independent experiments.

Estrogen inhibition of bone resorption by purified rabbit osteoclasts. Purified osteoclasts were incubated with medium (A) lacking or (B) containing 0.1 nM E₂ for 24 h on dentine slices (×40). After removal of osteoclasts, resorption pits formed by osteoclasts were stained with acid hematoxylin and observed under a light microscope. (C) Inhibitory effects of E₂ on osteoclast-mediated bone resorption. Purified osteoclasts were cultured on dentine slices (150 cells/slice) in medium (Con) or in medium containing 0.001–1 nM E₂ (E₂) or 1 nM salmon calcitonin (CT). Osteoclastic bone resorption activity was measured in terms of pit area formed by purified osteoclasts after 24 h of incubation. Pit area per pit number is also indicated (inset). Values are means ± SD, n = 4. *P <0.05, **P <0.01, ***P <0.005 compared with control groups. Data are representatives of those obtained in three additional independent experiments.