Table 1.
Proliferative Response and Cytokine Profile of PjE-specific CD8+ T Cell Clones from Atopic (A) and Nonatopic (B) Individuals
| 3H-TdR | IL-4 | IL-5 | IFN-γ | 3H-TdR | IL-4 | IL-5 | IFN-γ | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| cpm × 10−3 | pg/ml | pg/ml | pg/ml | cpm × 10−3 | pg/ml | pg/ml | pg/ml | |||||||||||
| Donor A | Donor B | |||||||||||||||||
| P4.2 | 30.5* | 537‡ | 746‡ | >4,000* | CP.27 | 23.4 | <20 | <20 | 2,395 | |||||||||
| P4.4 | 23.5 | <20 | <20 | >4,000 | CP.28 | 18.7 | 35 | <20 | >4,000 | |||||||||
| P4.6 | 33.9 | 222 | 959 | >4,000 | CP.31 | 14.6 | <20 | <20 | 3,000 | |||||||||
| P4.15 | 27.3 | 356 | <20 | >4,000 | CP.38 | 25.6 | <20 | <20 | 2,610 | |||||||||
| P4.16 | 23.9 | 232 | <20 | 2,821 | CP.44 | 10.7 | ND | ND | ND | |||||||||
| P4.17 | 42.3 | 80 | 543 | >4,000 | CP.45 | 7.4 | ND | ND | ND | |||||||||
| P4.26 | 44.3 | 1,378 | 1,300 | 3,394 | CP.53 | 26.0 | ND | ND | ND | |||||||||
| P4.27 | 29.0 | 664 | 319 | >4,000 | CP.56 | 10.0 | ND | ND | ND | |||||||||
| P4.33 | 30.3 | 518 | >2,000 | 2,624 | CP.66 | 27.8 | <20 | <20 | >4,000 | |||||||||
| P4.52 | 36.1 | 162 | <20 | 3,359 | CP.68 | 69.2 | 102 | <20 | >4,000 |
*
T cells were cultured with PjE (50 μg/ml) and autologous B-LCL. Proliferative response was measured on day 3. Background response in the absence of antigen for all clones was <10−3.
‡
T cells were stimulated with 10−7 M PMA + 0.2 μg/ml mAb TR66 (anti-CD3). After 40 h supernatants were collected and tested for cytokines by ELISA.