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. 1997 Sep 15;186(6):859–865. doi: 10.1084/jem.186.6.859

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(A) Freshly isolated, unrestimulated, peptide-specific, memory CD8⁺ T cells secrete IFN-γ within 6 h of antigen contact. Enumeration of IFN-γ SFCs in a 6 h ELISPOT assay performed with freshly isolated PBMC from donor SM (class I HLA haplotype: A2.01, A24; B44, B14). The HLA-A2.01–restricted peptide M1 58–66 was used at a final concentration of 2 μM. Mean values from duplicate wells are shown. (B) Irrelevant peptides do not induce IFN-γ SFCs. Using fresh PBMCs from HIV-uninfected donor SC (class I HLA haplotype: A2.01, A28; B7, B14) in a 6 h ELISPOT assay, quantitation of IFN-γ SFCs shows a response specific for the influenza HLA-A2.01–restricted peptide M1 58–66 compared with no response to the HLA-A2.01–restricted peptide from the HIV GAG protein, GAG 77-85 (SLYNTVATL).

(A) Freshly isolated, unrestimulated, peptide-specific, memory CD8⁺ T cells secrete IFN-γ within 6 h of antigen contact. Enumeration of IFN-γ SFCs in a 6 h ELISPOT assay performed with freshly isolated PBMC from donor SM (class I HLA haplotype: A2.01, A24; B44, B14). The HLA-A2.01–restricted peptide M1 58–66 was used at a final concentration of 2 μM. Mean values from duplicate wells are shown. (B) Irrelevant peptides do not induce IFN-γ SFCs. Using fresh PBMCs from HIV-uninfected donor SC (class I HLA haplotype: A2.01, A28; B7, B14) in a 6 h ELISPOT assay, quantitation of IFN-γ SFCs shows a response specific for the influenza HLA-A2.01–restricted peptide M1 58–66 compared with no response to the HLA-A2.01–restricted peptide from the HIV GAG protein, GAG 77-85 (SLYNTVATL).